Specific miRNA Stabilization by Gld2-Catalyzed Monoadenylation

Andrea D'Ambrogio, Weifeng Gu, Tsuyoshi Udagawa, Craig C. Mello, Joel D. Richter

研究成果: Article査読

82 被引用数 (Scopus)

抄録

MicroRNAs (miRNAs) are small, noncoding RNAs that inhibit translation and promote mRNA decay. The levels of mature miRNAs are the result of different rates of transcription, processing, and turnover. The noncanonical polymerase Gld2 has been implicated in the stabilization of miR-122, possibly through catalyzing 3@ monoadenylation; however, there is little evidence that this relationship is one of cause and effect. Here, we biochemically characterize Gld2@s involvement in miRNA monoadenylation and its effect on miRNA stability. We find that Gld2 directly monoadenylates and stabilizes specific miRNA populations in human fibroblasts and that sensitivity to monoadenylation-induced stability depends on nucleotides in the miRNA 3@ end. These results establish a mechanism of miRNA stability and resulting posttranscriptional gene regulation.

本文言語English
ページ(範囲)1537-1545
ページ数9
ジャーナルCell Reports
2
6
DOI
出版ステータスPublished - 2012 12月 27
外部発表はい

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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