The Fab′ fragment of a monoclonal antibody (mAb) directed to CD3 (a portion of the T cell receptor) and the Fab′ or F(ab′)2 fragment of an mAb to HIV were combined to generate bifunctional antibody (BFA) consisting of antiCD3-Fab′ conjugated with anti-HIV-Fab′ (Fab′/Fab′) or antiCD3-Fab′ conjugated with anti-HIV-F(ab′)2 (Fab′/F(ab′)2), respectively. In the presence of these BFA, HIV-infected target cells were cytolysed by peripheral blood lymphocytes. Treatment of lymphocytes with Fab′/F(ab′)2 type BFA rendered the lymphocytes significantly cytotoxic to HIV-infected target cells. Since BFA-armed lymphocytes can react on HIV-infected cells regardless of histocompatibility, lymphocytes from healthy donors could be armed with BFA for treatment of HIV-infected patients including those who do not exhibit histocompatibility with the lymphocyte donor.
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