Spatiotemporal expression of four isoforms of Ca2+/calmodulin-dependent protein kinase I in brain and its possible roles in hippocampal dendritic growth

Akifumi Kamata, Hiroyuki Sakagami, Hiroshi Tokumitsu, Yuji Owada, Kohji Fukunaga, Hisatake Kondo

研究成果: Article査読

20 被引用数 (Scopus)

抄録

Among multifunctional Ca2+/calmodulin-dependent protein kinases (CaMKs), CaMKI has been shown to comprise a family of four structurally related isoforms (α, β, γ, and δ) encoded by separate genes with abundant expression in mature brain. In this study, we first examined the developmental gene expression of the four isoforms of CaMKI in mouse brain with special attention to the hippocampal formation by in situ hybridization analysis. The four isoforms of CaMKI were found to exhibit distinct spatiotemporal expression during neuronal development. We also examined the functional involvement of CaMKI in the dendritic formation of cultured hippocampal neurons. The overexpression of kinase-dead mutants of CaMKI reduced the average dendritic length of the transfected neurons without any significant effects on the number of primary dendrites and the branching index. Our present findings provide the detailed anatomical information on the developmental expression of the four isoforms of CaMKI in mouse brain, which represents the possible functional involvement of CaMKI in the basal dendritic growth of hippocampal neurons.

本文言語English
ページ(範囲)86-97
ページ数12
ジャーナルNeuroscience Research
57
1
DOI
出版ステータスPublished - 2007 1月

ASJC Scopus subject areas

  • 神経科学(全般)

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