Somatic mutation in autoantibody-associated V(H) genes of circulating IgM+IgD+ B cells

Yasuhiko Munakata, Shinichiro Saito, Atsushi Hoshino, Tai Muryoi, Yasuhiko Hirabayashi, Shinobu Shibata, Toshihiko Miura, Tomonori Ishii, Tadao Funato, Takeshi Sasaki

研究成果: Article査読

11 被引用数 (Scopus)

抄録

Naive B cells expressing IgM and IgD on their surface have no or little somatic mutations in V genes. We have demonstrated that the human IgM+IgD+B cell clone (0-81), which expresses nephritogenic idiotypes, produces IgM anti-DNA antibodies which show monospecificity to DNA. Using a DNA probe which specifically links to the V(H) gene of antibody 0-81, we identified the counterpart germ-line V gene of 0-81, V3-7, which appears to be used by pathogenic autoantibodies in humans. Clone 0-81, which may belong to naive B cells in terms of cell phenotype, uses a somatically mutated V3-7 gene. We further studied DNA sequences of V3-7 genes in circulating IgM+IgD+B cells from normal subjects and patients with systemic lupus erythematosus (SLE). The results revealed that rearranged V3-7 genes in IgM+IgD+B cells from patients with SLE contained somatically mutated sequences at significantly increased frequencies. These data indicate an abnormal maturation of B cells in autoimmune states that may be associated with an escape of self-reactive B cells from the elimination process in the germinal center.

本文言語English
ページ(範囲)1435-1444
ページ数10
ジャーナルEuropean Journal of Immunology
28
5
DOI
出版ステータスPublished - 1998 5月
外部発表はい

ASJC Scopus subject areas

  • 免疫アレルギー学
  • 免疫学

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