@article{3d9f55d7efc84e4d9627e0f9bf549069,
title = "Solution structure of the homodimeric core domain of Escherichia coli histidine kinase EnvZ",
abstract = "Escherichia coli osmosensor EnvZ is a protein histidine kinase that plays a central role in osmoregulation, a cellular adaptation process involving the His-Asp phosphorelay signal transduction system. Dimerization of the transmembrane protein is essential for its autophosphorylation and phosphorelay signal transduction functions. Here we present the NMR-derived structure of the homodimeric core domain (residues 223-289) of EnvZ that includes His 243, the site of autophosphorylation and phosphate transfer reactions. The structure comprises a four-helix bundle formed by two identical helix-turn-helix subunits, revealing the molecular assembly of two active sites within the dimeric kinase.",
author = "C. Tomomori and T. Tanaka and R. Dutta and H. Park and Saha, {S. K.} and Y. Zhu and R. Ishima and D. Liu and Tong, {K. I.} and H. Kurokawa and H. Qian and M. Inouye and M. Ikura",
note = "Funding Information: We thank L. Kay for providing NMR pulse sequences, D. Garrett for helpful instructions on PIPP/STAPP, M. Osawa for providing software used in structure calculation, K. Yap for calculation of interhelical angles, and S. Bagby for critical reading of the manuscript. This work was supported by grants to T.T. from JSPS, to M. Inouye from the NIH, and to M. Ikura from the Howard Hughes Medical Institute. R.I. and D.L. acknowledge HFSP postdoctoral fellowships, and C.T. a postgraduate fellowship from the Ministry of Education, Science and Culture of Japan and from the TARA, University of Tsukuba. M. Ikura is an HHMI International Research Scholar and a Medical Research Council of Canada Scientist. Funding Information: We thank L. Pascoli and M. Hou for assistance with crystallization and J. Quintana and D. Keane for assistance with data collection. This work was supported by a grant from the NIH (to A.C.R.), by funds from the ALS Association (A.C.R.), by funds from the Robert H. Lurie Cancer Center (A.C.R.), by a grant from the NIH (to T.V.O.), by a supplement from NIGMS to this same grant (to A.C.R. and T.V.O.), by funds from the ALS Association (T.V.O.), and by an NIH NRSA Training Grant (R.A.P.). The DND-CAT Synchrotron Research Center at the Advanced Photon Sourceis supported by the E.I. Dupont de Nemours & Co., The Dow Chemical Company, the NSF and the State of Illinois.",
year = "1999",
doi = "10.1038/11495",
language = "English",
volume = "6",
pages = "729--734",
journal = "Nature Structural Biology",
issn = "1545-9993",
publisher = "Nature Publishing Group",
number = "8",
}