Sodium butyrate-induced liver-type alkaline phosphatase activity in a small intestinal epithelial cell line, IEC6

Kouhei Fukushima, Iwao Sasaki, Hideaki Hasegawa, Ken Ichi Takahashi, Hiroo Naito, Yuji Funayama, Seiki Matsuno

研究成果: Article査読

26 被引用数 (Scopus)

抄録

Sodium butyrate is a well-recognized differentiating agent inducing alkaline phosphatase activity, one of the epithelial differentiation markers. When IEC6 cells, a nontransformed, small intestinal epithelial cell line, were cultured with butyrate, this substrate induced alkaline phosphate activity in a time- and dose-dependent fashion. However, the type of isoenzyme involved was a liver-type, not an intestinal-type. Electron microscopy revealed that the induced activity was strictly localized in the cytosol and not on the plasma membrane. However, disaccharidase activities, another kind of differentiation marker, were also enhanced by sodium butyrate. In addition, the positive cells demonstrating the presence of alkaline phosphatase activity were preferentially observed in tubular structures. These data show that butyrate-induced alkaline phosphatase activity is closely associated with differentiation-like phenomena in IEC6 cells, although the type of isoenzyme and cellular localization of the activity are different from those observed in mucosa.

本文言語English
ページ(範囲)1116-1123
ページ数8
ジャーナルDigestive Diseases and Sciences
43
5
DOI
出版ステータスPublished - 1998
外部発表はい

ASJC Scopus subject areas

  • 生理学
  • 消化器病学

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