Sodium and chloride ion-dependent transport of β-alanine across the blood-brain barrier

Junko Komura, Ikumi Tamai, Mizuho Senmaru, Tetsuya Terasaki, Yoshimichi Sai, Akira Tsuji

研究成果: Article査読

29 被引用数 (Scopus)

抄録

The characteristics of β-alanine transport at the blood-brain barrier were studied by using primary cultured bovine brain capillary endothelial cells. Kinetic analysis of the β-[3H]alanine transport indicated that the transporter for β-alanine functions with K(t) of 25.3 ± 2.5 μM and J(max) of 6.90 ± 0.48 nmol/30 min/mg of protein in the brain capillary endothelial cells. β-[3H]Alanine uptake is mediated by an active transporter, because metabolic inhibitors (2,4-dinitrophenol and NaN3) and low temperature reduced the uptake significantly. Furthermore, the uptake of β-[3H]alanine required Na+ and Cl- in the external medium. Stoichiometric analysis of the transport demonstrated that two sodium ions and one chloride ion are associated with one β-alanine molecule. The Na+ and Cl- dependent uptake of β-[3H]alanine was stimulated by a valinomycin-induced inside-negative K+-diffusion potential. β-Amino acids (β-alanine, taurine, and hypotaurine) inhibited strongly the uptake of β-[3H]alanine, whereas α- and γ-amino acids had little or no inhibitory effect. In ATP-depleted cells, the uptake of β[3H]alanine was stimulated by preloading of β-alanine or taurine but not L-leucine. These results show that β-alanine is taken up by brain capillary endothelial cells, via the secondary active transport mechanism that is common to β-amino acids.

本文言語English
ページ(範囲)330-335
ページ数6
ジャーナルJournal of Neurochemistry
67
1
DOI
出版ステータスPublished - 1996 7

ASJC Scopus subject areas

  • Biochemistry
  • Cellular and Molecular Neuroscience

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