SNARE conformational changes that prepare vesicles for exocytosis

Noriko Takahashi, Hiroyasu Hatakeyama, Haruo Okado, Jun Noguchi, Mitsuyo Ohno, Haruo Kasai

研究成果: Article査読

59 被引用数 (Scopus)


When cells release hormones and neurotransmitters through exocytosis, cytosolic Ca2+ triggers the fusion of secretory vesicles with the plasma membrane. It is well known that this fusion requires assembly of a SNARE protein complex. However, the timing of SNARE assembly relative to vesicle fusion - essential for understanding exocytosis - has not been demonstrated. To investigate this timing, we constructed a probe that detects the assembly of two plasma membrane SNAREs, SNAP25 and syntaxin-1A, through fluorescence resonance energy transfer (FRET). With two-photon imaging, we simultaneously measured FRET signals and insulin exocytosis in β cells from the pancreatic islet of Langerhans. In some regions of the cell, we found that the SNARE complex was preassembled, which enabled rapid exocytosis. In other regions, SNARE assembly followed Ca2+ influx, and exocytosis was slower. Thus, SNARE proteins exist in multiple stable preparatory configurations, from which Ca2+ may trigger exocytosis through distinct mechanisms and with distinct kinetics.

ジャーナルCell Metabolism
出版ステータスPublished - 2010 7月 7

ASJC Scopus subject areas

  • 生理学
  • 分子生物学
  • 細胞生物学


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