抄録
Platelets play essential roles in hemostasis and thrombosis by aggregating with each other. However, the molecular mechanism governing platelet aggregation is not yet fully understood. Here, we established an assay system using platelets permeabilized with streptolysin-O to analyze mechanism of the thrombin-induced aggregation, focusing upon a controversial issue in the field whether small GTPase Rho regulates the aggregation. Incubation of the permeabilized platelets with Rho GDP-dissociation inhibitor, an inhibitory regulator for Rho family GTPases, extracted Rho family proteins extensively from the plasma and intracellular membranes, and inhibited the thrombin-induced aggregation. Incubation of the permeabilized platelets with botulinum exoenzyme C3, which specifically inhibits Rho function by ADP-ribosylating it, abolished the thrombin-induced aggregation. Thus, Rho is involved in thrombin-induced aggregation of platelets.
本文言語 | English |
---|---|
ページ(範囲) | 970-975 |
ページ数 | 6 |
ジャーナル | Biochemical and biophysical research communications |
巻 | 280 |
号 | 4 |
DOI | |
出版ステータス | Published - 2001 |
外部発表 | はい |
ASJC Scopus subject areas
- 生物理学
- 生化学
- 分子生物学
- 細胞生物学