Single‐channel recordings of chloride currents in primary cultured Drosophila neurons

Daisuke Yamamoto, Nobuyuki Suzuki

    研究成果: Article査読

    6 被引用数 (Scopus)

    抄録

    Single‐chloride‐channel currents were recorded from primary cultured Drosophila neurons by means of the gigaohm‐seal patch‐clamp technique. Small inward‐going current channels were observed in excised inside‐out patches with the external face of the membrane exposed to bathing solutions devoid of K+, Na+, and Ca2+. The inward current was affected by changing the anions but not the cations bathing the cytoplasmic face of the patch. Complete replacement of CI by glutamate eliminated the current. The current was maintained with intracellular solutions containing NO3 in place of CI. The single‐channel conductance was estimated to be 7 ps with CI, and 11 ps with NO3 at 10°C. Possible functions of this anion‐selective channel have been discussed.

    本文言語English
    ページ(範囲)151-158
    ページ数8
    ジャーナルArchives of Insect Biochemistry and Physiology
    6
    3
    DOI
    出版ステータスPublished - 1987 11月

    ASJC Scopus subject areas

    • 生理学
    • 生化学
    • 昆虫科学

    フィンガープリント

    「Single‐channel recordings of chloride currents in primary cultured Drosophila neurons」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

    引用スタイル