Single-protein study of photoresistance of pigment-protein complex in lipid bilayer

Daisuke Uchiyama, Hajime Hoshino, Kohei Otomo, Taro Kato, Ken Ichi Onda, Akira Watanabe, Hiroyuki Oikawa, Satoru Fujiyoshi, Michio Matsushita, Mamoru Nango, Natsuko Watanabe, Ayumi Sumino, Takehisa Dewa

研究成果: Article査読

4 被引用数 (Scopus)

抄録

Photoresistance of a pigment-binding membrane protein, light-harvesting 2 (LH2) complex from the photosynthetic bacterium, Rhodopseudomonas acidophila, was investigated by fluorescence of single LH2 complexes at a temperature of 296 K. Before irreversibly stopping fluorescence, a single LH2 complex in phospholipid bilayer of dimyristoylphosphatidylcholine (DMPC) emitted on average 4 times more fluorescence photons than a complex in detergent micelle of octylglucopyranoside (OG). Fluorescence-excitation spectrum of single LH2 complexes taken at 5 K showed that the LH2 complex is structurally less perturbed in DMPC bilayer than in OG micelle, suggesting that structural instability reduces photoresistance of LH2.

本文言語English
ページ(範囲)135-137
ページ数3
ジャーナルChemical Physics Letters
511
1-3
DOI
出版ステータスPublished - 2011 7月 26
外部発表はい

ASJC Scopus subject areas

  • 物理学および天文学(全般)
  • 物理化学および理論化学

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