Single-molecule analysis of the actomyosin motor using nano-manipulation

Akihiko Ishijima, Yoshie Harada, Hiroaki Kojima, Takashi Funatsu, Hideo Higuchi, Toshio Yanagida

研究成果: Article査読

173 被引用数 (Scopus)

抄録

The elementary events in energy transduction by the actomyosin motor, driven by ATP hydrolysis, were directly recorded from multiple and single molecules using a recently developed technique for nano-manipulation of single actin filaments by a microneedle. In order to avoid the effects of random orientation of myosin and association of myosin with an artificial substrate in the surface motility assay, we used single myosin-rod cofilaments with various ratios. Distinct actomyosin attachment, force generation (the power stroke) and detachment events were detected at a very low myosin: rod ratio. At high load, one power stroke generated 5 - 6 pN peak force and 2.3 pN force averaged over the cycle, which were compatible with those deduced from noise analysis of force fluctuations caused by multiple molecules. As the load was reduced, the length of the power stroke increased. At near zero load, the length of a power stroke was approximately 17 nm. The results suggested that an ATPase cycle produces one power stroke at high load and many ones at low load.

本文言語English
ページ(範囲)1057-1063
ページ数7
ジャーナルBiochemical and biophysical research communications
199
2
DOI
出版ステータスPublished - 1994 3 15

ASJC Scopus subject areas

  • Biophysics
  • Biochemistry
  • Molecular Biology
  • Cell Biology

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