Simultaneous observation of individual ATPase and mechanical events by a single myosin molecule during interaction with actin

Akihiko Ishijima, Hiroaki Kojima, Takashi Funatsu, Makio Tokunaga, Hideo Higuchi, Hiroto Tanaka, Toshio Yanagida

研究成果: Article査読

420 被引用数 (Scopus)

抄録

We have developed a technique that allows mechanical and ligand-binding events in a single myosin molecule to be monitored simultaneously. We describe how steps in the ATPase reaction are temporally related to mechanical events at the single molecule level. The results show that the force generation does not always coincide with the release of bound nucleotide, presumably ADP. Instead the myosin head produces force several hundreds of milliseconds after bound nucleotide is released. This finding does not support the widely accepted view that force generation is directly coupled to the release of bound ligands. It suggests that myosin has a hysteresis or memory state, which stores chemical energy from ATP hydrolysis.

本文言語English
ページ(範囲)161-171
ページ数11
ジャーナルCell
92
2
DOI
出版ステータスPublished - 1998 1 23

ASJC Scopus subject areas

  • 生化学、遺伝学、分子生物学(全般)

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