Simultaneous determination of guanidinosuccinic acid and guanidinoacetic acid in urine using high performance liquid chromatography/tandem mass spectrometry

Daisuke Saigusa, Naoto Suzuki, Mai Takahashi, Kanako Shiba, Satoshi Tanaka, Takaaki Abe, Takanori Hishinuma, Yoshihisa Tomioka

研究成果: Article査読

14 被引用数 (Scopus)

抄録

We present a method for the simultaneous determination of guanidinosuccinic acid (GSA) and guanidinoacetic acid (GAA) from urine by protein precipitation and liquid chromatography/tandem mass spectrometry. The chromatographic separation was performed using a cation exchange column with an elution gradient of 0.1mM and 20mM ammonium acetate buffers. GSA was detected with the mass spectrometer in negative ion mode monitoring at m/z 174.1, and GAA, creatinine, arginine, and homoarginine were in positive ion mode monitoring at m/z 118.1, 114.1, 175.1, and 189.1, respectively. As an internal standard, l-arginine-13C6 hydrochloride and creatinine-d3 (methyl-d3) were used. The calibration ranges were 0.50-25.0μgmL-1, and good linearities were obtained for all compounds (r>0.999). The intra- and inter-assay accuracies (expressed as recoveries) and precisions at three concentration levels (1.00, 5.00 and 25.0μgmL-1) were better than 83.8% and 7.41%, respectively. The analytical performance of the method was evaluated by determination of the compounds in urine from male C57BL/J Iar db/db diabetes mellitus (DM) mice. The values of GSA and GAA corrected by the ratios of the individual compounds to creatinine were significantly increased in DM mice compared with control mice. These results indicated that the newly developed method was useful for determining urinary guanidino compounds and metabolites of arginine.

本文言語English
ページ(範囲)169-175
ページ数7
ジャーナルAnalytica Chimica Acta
677
2
DOI
出版ステータスPublished - 2010 9

ASJC Scopus subject areas

  • 分析化学
  • 生化学
  • 環境化学
  • 分光学

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