抄録
In F1 hybrid breeding of Brassica vegetables utilizing the self-incompatibility system, identification of S genotypes in breeding lines is required. In the present study, we developed S-tester lines of 87 S haplotypes, i. e., 42 S haplotypes in B. rapa and 45 S haplotypes in B. oleracea. With these materials, we established a simple, efficient, and reliable dot-blot technique for S genotyping for 40 S haplotypes of B. rapa and and 33 of B. oleracea using allele-specific oligonucleotide probes and allele-specific primer pairs designed from sequences of each SP11 allele. In this method, DNA fragments amplified using multiplex primer pairs with digoxigenin-dUTP were hybridized with dot-blotted allele-specific oligonucleotide probes with distinct signals. In addition, we developed a screening method for identification of plants harboring a particular S haplotype using a labeled allele-specific oligonucleotide probe. This method is considered to be useful for purity testing of F1 hybrid seeds.
本文言語 | English |
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ページ(範囲) | 1-12 |
ページ数 | 12 |
ジャーナル | Molecular Breeding |
巻 | 28 |
号 | 1 |
DOI | |
出版ステータス | Published - 2011 6月 |
ASJC Scopus subject areas
- バイオテクノロジー
- 分子生物学
- 農業および作物学
- 遺伝学
- 植物科学