TY - JOUR
T1 - Short-term feeding of conjugated linoleic acid does not induce hepatic steatosis in C57BL/6J mice
AU - Wang, Yu Ming
AU - Nagao, Koji
AU - Ujino, Yoko
AU - Sakata, Kotaro
AU - Higa, Kouki
AU - Inoue, Nao
AU - Yanagita, Teruyoshi
PY - 2005/12
Y1 - 2005/12
N2 - We investigated the effect of short-term feeding of conjugated linoleic acid (CLA) on adipose tissue weights, liver weight, hepatic lipid metabolism, and serum lipoprotein profiles in C57BL/6J mice. Mice were fed semi-synthetic diets containing either 6% high-linoleic safflower oil (HL-SAF) or 4% HL-SAF+2% CLA for 1 wk. Short-term feeding of CLA showed an anti-obesity effect without inducing hepatomegaly in mice. In addition to the decline of hepatic triglyceride concentration, significant inhibition of Δ9 desaturation of fatty acid in the total liver lipids was found in CLA-fed mice. The CLA diet significantly increased the activities of peroxisomal β-oxidation and decreased the activities of diacylglycerol acyltransferase, a triglyceride synthesis-related enzyme, in the liver. Moreover, serum lipoprotein profiles of CLA-fed mice showed preferable changes in the atherogenic indices. However, serum leptin and adiponectin were drastically decreased by CLA feeding, suggesting that prolonged administration of CLA would induce further decrease of serum adipocytokine levels, which may be a cause of lipodystrophy in mice. These results show that short-term feeding of CLA does not induce adverse effect in C57EL/6J mice.
AB - We investigated the effect of short-term feeding of conjugated linoleic acid (CLA) on adipose tissue weights, liver weight, hepatic lipid metabolism, and serum lipoprotein profiles in C57BL/6J mice. Mice were fed semi-synthetic diets containing either 6% high-linoleic safflower oil (HL-SAF) or 4% HL-SAF+2% CLA for 1 wk. Short-term feeding of CLA showed an anti-obesity effect without inducing hepatomegaly in mice. In addition to the decline of hepatic triglyceride concentration, significant inhibition of Δ9 desaturation of fatty acid in the total liver lipids was found in CLA-fed mice. The CLA diet significantly increased the activities of peroxisomal β-oxidation and decreased the activities of diacylglycerol acyltransferase, a triglyceride synthesis-related enzyme, in the liver. Moreover, serum lipoprotein profiles of CLA-fed mice showed preferable changes in the atherogenic indices. However, serum leptin and adiponectin were drastically decreased by CLA feeding, suggesting that prolonged administration of CLA would induce further decrease of serum adipocytokine levels, which may be a cause of lipodystrophy in mice. These results show that short-term feeding of CLA does not induce adverse effect in C57EL/6J mice.
KW - C57BL/6J mice
KW - Conjugated linoleic acid
KW - Diacylglycerol acyltransferase
KW - Hepatic steatosis
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U2 - 10.3177/jnsv.51.440
DO - 10.3177/jnsv.51.440
M3 - Article
C2 - 16521704
AN - SCOPUS:31444437257
VL - 51
SP - 440
EP - 444
JO - The Journal of vitaminology
JF - The Journal of vitaminology
SN - 0301-4800
IS - 6
ER -