Sequence comparisons among dispersed members of the Brassica S multigene family in an S9 genome

N. Kai, G. Suzuki, M. Watanabe, A. Isogai, K. Hinata

研究成果: Article査読

16 被引用数 (Scopus)

抄録

Self-incompatibility (SI) systems prevent self-pollination and promote outbreeding. In Brassica, the SI genes SLG (for S-locus glycoprotein) and SRK (for S-receptor kinase) are members of the S multigene family, which share the SLG-like domain (S domain), which encodes a putative receptor. We have cloned members of the S multigene family from the S9 haplotype of B. campestris (syn. rapa). In addition, eight distinct genomic regions harboring 10 SLG/SRK-like genes were characterized in the present study. Sequence analysis revealed two novel SRK-like genes, BcRK3 and BcRK6 (for B. campestris receptor kinases 3 and 6, respectively). Other genes that were characterized included SFR2 (for S gene family receptor 2), SLR2 (for S locus related gene 2), and a pseudogene. Based on phylogenetic analysis of the nucleotide sequences of the S domain regions, SLG and SRK appear to be distinct from other members of the S multigene family. Linkage analysis showed that most members of the S multigene family are dispersed in the Brassica genome, and that SLR1 (S locus related gene 1) is not linked to the SLR2 in B. campestris.

本文言語English
ページ(範囲)526-534
ページ数9
ジャーナルMolecular and General Genetics
265
3
DOI
出版ステータスPublished - 2001 1月 1

ASJC Scopus subject areas

  • 遺伝学

フィンガープリント

「Sequence comparisons among dispersed members of the Brassica S multigene family in an S9 genome」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル