Separation of proteins BY continuous isoelectric focusing

Shuang Ning Zheng, Toshikuni Yonemoto, Teiriki Tadaki

研究成果: Article査読

1 被引用数 (Scopus)

抄録

An experimental investigation of continuous electrophoretic separation of bovine serum albumin from bovine hemoglobin was carried out. The separation behavior was analyzed with the model equations presented in our previous paper. We examined the effect of operating parameters such as applied voltage, residence time and the buffer added to feed solution including carrier ampholyte, on the basis of experimental results and theoretical predications, and showed that 1) an optimum applied voltage exists for the electrophoretic separation process of proteins, and 2) the buffer concentration greatly influences the pH distribution and the separation behavior of proteins.

本文言語English
ページ(範囲)471-476
ページ数6
ジャーナルJOURNAL of CHEMICAL ENGINEERING of JAPAN
24
4
DOI
出版ステータスPublished - 1991

ASJC Scopus subject areas

  • 化学 (全般)
  • 化学工学(全般)

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