Semaphorin controls epidermal morphogenesis by stimulating mRNA translation via eIF2α in Caenorhabditis elegans

Akira Nukazuka, Hajime Fujisawa, Toshifumi Inada, Yoichi Oda, Shin Takagi

研究成果: Article

29 引用 (Scopus)

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Conserved semaphorin-plexin signaling systems govern various aspects of animal development, including axonal guidance in vertebrates and epidermal morphogenesis in Caenorhabditis elegans. Here we provide in vivo evidence that stimulation of mRNA translation via eukaryotic initiation factor 2α (eIF2α) is an essential downstream event of semaphorin signaling in C. elegans. In semaphorin/plexin mutants, a marked elevation in the phosphorylation of eIF2α is observed, which causes translation repression and is causally related to the morphological epidermal phenotype in the mutants. Conversely, removal of constraints on translation by genetically reducing the eIF2α phosphorylation largely bypasses requirement for the semaphorin signal in epidermal morphogenesis. We also identify an actin-depolymerizing factor/cofilin, whose expression in the mutants is predominantly repressed, as a major translational target of semaphorin signaling. Thus, our results reveal a physiological significance for translation of mRNAs for cytoskeletal regulators, linking environmental cues to cytoskeletal rearrangement during cellular morphogenesis in vivo.

元の言語English
ページ(範囲)1025-1036
ページ数12
ジャーナルGenes and Development
22
発行部数8
DOI
出版物ステータスPublished - 2008 4 15
外部発表Yes

ASJC Scopus subject areas

  • Genetics
  • Developmental Biology

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