Self-formation of bilayer lipid membranes on agarose-coated silicon surfaces studied by simultaneous electrophysiological and surface infrared spectroscopic measurements

Ayumi Hirano-Iwata, Azusa Oshima, Kota Onodera, Kouji Aoto, Tasuku Taira, Ryo Taro Yamaguchi, Yasuo Kimura, Michio Niwano

研究成果: Article査読

5 被引用数 (Scopus)

抄録

Self-formation process of bilayer lipid membranes (BLMs) cushioned on agarose-coated Si surfaces was in situ monitored by simultaneous electrophysiological and infrared absorption spectroscopic (IRAS) measurements using IRAS with the multiple internal reflection geometry. IRAS signals corresponding to self-thinning of lipid solution to form BLMs were demonstrated. It was found that the appearance of IRAS bands due to C=O modes of phosphstidylcholine is related to formation of BLMs with a gigaohm seal. The functionality of the present BLM system was also demonstrated by incorporating gramicidin into the BLMs and recording its channel activities.

本文言語English
論文番号243906
ジャーナルApplied Physics Letters
94
24
DOI
出版ステータスPublished - 2009

ASJC Scopus subject areas

  • 物理学および天文学(その他)

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