Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template

Takeo Miyake, Takashi Tanii, Koichi Kato, Tamotsu Zako, Takashi Funatsu, Iwao Ohdomari

    研究成果: Article査読

    13 被引用数 (Scopus)

    抄録

    Protein nanopatterning techniques to fulfil the requirement for reducing nonspecific adsorption are demonstrated. A target protein is selectively immobilized on a hydroxy-terminated self-assembled monolayer template, the pattern of which is modified with streptavidin used as the intermediating molecule. 3-aminopropyltrimethoxysilane (APTES) used as the intermediating molecule induces nonspecific adsorption of proteins due to nonspecific adsorption of APTES itself. The data indicate that reducing nonspecific adsorption of both the target protein and the intermediating molecule is important for selectivity improvement in protein patterning. As a result of the refinement, a protein nanopattern of 250 nm in diameter has been fabricated.

    本文言語English
    論文番号305304
    ジャーナルNanotechnology
    18
    30
    DOI
    出版ステータスPublished - 2007 8 8

    ASJC Scopus subject areas

    • バイオエンジニアリング
    • 化学 (全般)
    • 材料科学(全般)
    • 材料力学
    • 機械工学
    • 電子工学および電気工学

    フィンガープリント

    「Selectivity improvement in protein nanopatterning with a hydroxy-terminated self-assembled monolayer template」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

    引用スタイル