Selective labeling of proteins on living cell membranes using fluorescent nanodiamond probes

Shingo Sotoma, Jun Iimura, Ryuji Igarashi, Koichiro M. Hirosawa, Hidenori Ohnishi, Shin Mizukami, Kazuya Kikuchi, Takahiro K. Fujiwara, Masahiro Shirakawa, Hidehito Tochio

研究成果: Article査読

21 被引用数 (Scopus)

抄録

The impeccable photostability of fluorescent nanodiamonds (FNDs) is an ideal property for use in fluorescence imaging of proteins in living cells. However, such an application requires highly specific labeling of the target proteins with FNDs. Furthermore, the surface of unmodified FNDs tends to adsorb biomolecules nonspecifically, which hinders the reliable targeting of proteins with FNDs. Here, we combined hyperbranched polyglycerol modification of FNDs with the -lactamase-tag system to develop a strategy for selective imaging of the protein of interest in cells. The combination of these techniques enabled site-specific labeling of Interleukin-18 receptor alpha chain, a membrane receptor, with FNDs, which eventually enabled tracking of the diffusion trajectory of FND-labeled proteins on the membrane surface.

本文言語English
論文番号56
ジャーナルNanomaterials
6
4
DOI
出版ステータスPublished - 2016 4月 1
外部発表はい

ASJC Scopus subject areas

  • 化学工学(全般)
  • 材料科学(全般)

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