Selective inactivation of elongation factor-2 (EF-2) in free adipocytes obtained by treating Sarcophaga larval fat bodies with chymotrypsin

Hajime Okuyama, Shoichiro Kurata, Ko Ichi Homma, Shunji Natori

研究成果: Article査読

抄録

The molecular mechanism(s) involved in the selective cessation of protein synthesis in free adipocytes obtained by treating fat bodies of Sarcophaga larvae with chymotrypsin were investigated (Okuyama, H., Kurata, S., Natori, S., 1994. Insect Biochem. Mol. Biol. 24, 525-530). Ribosomes prepared from these adipocytes were normal, but the activity of soluble factor(s) needed for protein synthesis was greatly reduced compared with the control fat bodies. From experiments using diphtheria toxin, we concluded that EF-2, but not other factors, was selectively inactivated in these free adipocytes.

本文言語English
ページ(範囲)301-307
ページ数7
ジャーナルInsect Biochemistry and Molecular Biology
28
5-6
DOI
出版ステータスPublished - 1998 5月
外部発表はい

ASJC Scopus subject areas

  • 生化学
  • 分子生物学
  • 昆虫科学

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