The molecular mechanism(s) involved in the selective cessation of protein synthesis in free adipocytes obtained by treating fat bodies of Sarcophaga larvae with chymotrypsin were investigated (Okuyama, H., Kurata, S., Natori, S., 1994. Insect Biochem. Mol. Biol. 24, 525-530). Ribosomes prepared from these adipocytes were normal, but the activity of soluble factor(s) needed for protein synthesis was greatly reduced compared with the control fat bodies. From experiments using diphtheria toxin, we concluded that EF-2, but not other factors, was selectively inactivated in these free adipocytes.
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