Selection of in vitro-matured porcine oocytes based on localization patterns of lipid droplets to evaluate developmental competence

Kou Hiraga, Yumi Hoshino, Kentaro Tanemura, Eimei Sato

研究成果: Article査読

6 被引用数 (Scopus)

抄録

Localization patterns of lipid droplets in the cytoplasm of porcine oocytes were evaluated as a novel marker for in vitro maturation (IVM) of oocytes with high developmental competence. Porcine oocytes were cultured in TCM-199, which is a complete synthetic medium, for 44 h at 38.5 C. Localization patterns were divided into 2 classes: lipid droplets localized uniformly in the whole cytoplasm (class I) and those that were centrally located (class II). After IVM in TCM-199, 60% of matured oocytes exhibited the class II pattern. To investigate the relation between the distribution of lipid droplets and the developmental rate of the oocyte, the developmental rates of class I and class II oocytes were compared after in vitro fertilization (IVF). Class II oocytes showed a significantly higher rate of blastocyst development than class I oocytes. These results suggest that porcine oocytes with high developmental competence can be selected based on the localization patterns of lipid droplets.

本文言語English
ページ(範囲)405-408
ページ数4
ジャーナルJournal of Reproduction and Development
59
4
DOI
出版ステータスPublished - 2013

ASJC Scopus subject areas

  • Animal Science and Zoology

フィンガープリント 「Selection of in vitro-matured porcine oocytes based on localization patterns of lipid droplets to evaluate developmental competence」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル