Secretion of calf chymosin from the filamentous fungus Aspergillus oryzae

Kozo Tsuchiya, Katsuya Gomi, Katsuhiko Kitamoto, Chieko Kumagai, Gakuzo Tamura

研究成果: Article査読

48 被引用数 (Scopus)

抄録

Active calf chymosin was secreted from Aspergillus oryzae transformants when the chymosin cDNA was expressed under the control of glucoamylase gene (glaA) promoter. Secreted prochymosin was autocatalytically activated to the chymosin (0.07- 0.16 mg/l). Western blot analysis showed that a secreted protein immunoreactive with an anti-chymosin antibody was of similar size to authentic chymosin. Northern blot analysis revealed that mRNA of the chymosin cDNA was expressed at as high level as that of the glaA gene. The size and the level of the transcript were different among transformants, due to the intergration position of the plasmid on the chromosome.

本文言語English
ページ(範囲)327-332
ページ数6
ジャーナルApplied Microbiology and Biotechnology
40
2-3
DOI
出版ステータスPublished - 1993 11月 1
外部発表はい

ASJC Scopus subject areas

  • バイオテクノロジー
  • 応用微生物学とバイオテクノロジー

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