Secretion and gene expression of secretory leukocyte protease inhibitor by human airway submucosal glands

Hiroki Saitoh, Tohru Masuda, Sanae Shimura, Toshiaki Fushimi, Kunio Shirato

研究成果: Article査読

36 被引用数 (Scopus)

抄録

Submucosal glands were isolated within 4 h of death from tracheae and bronchi obtained from autopsied lungs, and the secretory response of secretory leukocyte protease inhibitor (SLPI) was examined with ELISA and a secretory index. Although human neutrophil elastase (HNE) at low concentrations increased SLPI secretion above the control level (i.e., 149% of control level at 10-11 M), HNE at high concentrations significantly decreased it below the control level (i.e., 16% of control level at 10-7 M). The decrease in SLPI concentration was shown to result from the degradation of SLPI by excessive HNE. Methacholine induced significant secretion (i.e., 363% of control level at 10-5 M) that was abolished by both M1 and M3 receptor antagonists. A semiquantitative analysis of SLPI mRNA by RT-PCR and Southern blot showed that compared with the superficial epithelium, submucosal glands had a 30-fold or higher level of SLPI mRNA. Both HNE and methacholine significantly increased the level of SLPI mRNA in submucosal glands in a dose-dependent manner (i.e., 357% of control level at 10-7 M and 175% of control level at 10-5 M, respectively). These findings indicate that human airway submucosal glands can transcribe 30-fold or more SLPI mRNA than the superficial epithelium and that SLPI mRNA transcription and secretion are regulated by both HNE and muscarinic receptors.

本文言語English
ページ(範囲)L79-L87
ジャーナルAmerican Journal of Physiology - Lung Cellular and Molecular Physiology
280
1 24-1
DOI
出版ステータスPublished - 2001 1月

ASJC Scopus subject areas

  • 生理学
  • 呼吸器内科
  • 生理学(医学)
  • 細胞生物学

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