Screening of DNA aptamer which binds to α-synuclein

Kaori Tsukakoshi, Ryuichi Harada, Koji Sode, Kazunori Ikebukuro

研究成果: Article査読

31 被引用数 (Scopus)

抄録

α-Synuclein is a native, unfolded protein that causes several neurodegenerative diseases such as dementia with Lewy bodies and Parkinson's disease. We have now identified the first DNA aptamers against α-synuclein using native PAGE applied to the SELEX method. We call this aptamer "M5-15"; it is the α-synuclein-bound aptamer and was isolated after four cycles of screening. M5-15 is composed of three stem-loop structures that may play an important role in the binding to α-synuclein. Moreover, M5-15 specifically binds to the α-synuclein monomer and oligomer. We expect that this aptamer will become a useful tool in α-synuclein analysis and diagnosis.

本文言語English
ページ(範囲)643-648
ページ数6
ジャーナルBiotechnology Letters
32
5
DOI
出版ステータスPublished - 2010 2 1
外部発表はい

ASJC Scopus subject areas

  • Biotechnology
  • Bioengineering
  • Applied Microbiology and Biotechnology

フィンガープリント 「Screening of DNA aptamer which binds to α-synuclein」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

引用スタイル