Saturated fatty acids suppress adrenocorticotropic hormone (ACTH) release from rat anterior pituitary cells in vitro

Kazuo Katoh, Mami Asari, Hiroko Ishiwata, Yasuyuki Sasaki, Yoshiaki Obara

    研究成果: Article査読

    12 被引用数 (Scopus)

    抄録

    We studied whether fatty acids modify adrenocorticotropic hormone (ACTH) release induced by stimulation with corticotropin-releasing hormone (CRH) from rat anterior pituitary cells. Stimulation with CRH (0.01-100 nmol/l) significantly and concentration-dependently increased ACTH release, which was synergistically enhanced by the simultaneous stimulation with 1 nmol/l arginine-vasopressin. Addition of saturated fatty acids (butyrate, caprylate, laurate, palmitate and stearate) in a medium at 1 mmol/l, despite effects on the basal release, significantly reduced the ACTH release induced by CRH (1 nmol/l) stimulation. Caprylate suppressed ACTH release in a concentration- dependent manner. However, unsaturated C18 and C20 fatty acids (oleate, linolate, linolenate and arachidonate) at 1 mmol/l significantly increased the basal release, but none of them suppressed CRH (1 nmol/l)-induced ACTH release. In the presence of caprylate (1 mmol/l), CRH (1 nmol/l)-stimulated increase in cellular calcium ion concentration was diminished. From these results we conclude that saturated fatty acids have a suppressing effect on CRH-induced ACTH increase in primary cultured rat anterior pituitary cells.

    本文言語English
    ページ(範囲)357-364
    ページ数8
    ジャーナルComparative Biochemistry and Physiology - A Molecular and Integrative Physiology
    137
    2
    DOI
    出版ステータスPublished - 2004 2月

    ASJC Scopus subject areas

    • 生化学
    • 生理学
    • 分子生物学

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