Saturated fatty acids stimulate and insulin suppresses CIDE-A expression in bovine mammary epithelial cells

Tomo Yonezawa, Satoshi Haga, Yosuke Kobayashi, Kazuo Katoh, Yoshiaki Obara

    研究成果: Article査読

    13 被引用数 (Scopus)

    抄録

    Cell death-inducing DNA fragmentation factor-alpha-like effector A (CIDE-A) was first identified by its sequence homology with the N-terminal domain of DNA fragmentation factor (DFF). CIDE-A negatively regulates the activity of uncoupling protein 1 (UCP1) in brown adipose tissue. CIDE-A and UCP1 mRNA were detected by RT-PCR in cloned bovine mammary epithelial cells (bMEC) and lactating bovine mammary glands. Physiological concentrations of saturated fatty acids (stearate and palmitate), but not unsaturated fatty acids (oleate and linoleate) induced up-regulation of CIDE-A mRNA in bMEC. Treatment with insulin (5-10 ng/ml) induced down-regulation of CIDE-A and UCP1. The expression levels of CIDE-A and UCP1 mRNA in bovine mammary glands at various stages of the lactation cycle were determined by quantitative RT-PCR analysis. CIDE-A mRNA expression at peak lactation (2 months after parturition) was significantly higher than at dry off and non-pregnancy but not late lactation. These results suggest that CIDE-A and UCP1 are regulated by insulin and/or fatty acids in mammary epithelial cells and lactating mammary glands, and thereby play an important role in lipid and energy metabolism.

    本文言語English
    ページ(範囲)535-539
    ページ数5
    ジャーナルBiochemical and biophysical research communications
    384
    4
    DOI
    出版ステータスPublished - 2009 7月 10

    ASJC Scopus subject areas

    • 生物理学
    • 生化学
    • 分子生物学
    • 細胞生物学

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