Role of S4 segments and the leucine heptad motif in the activation of an L-type calcium channel

J. García, J. Nakai, K. Imoto, K. G. Beam

研究成果: Article査読

54 被引用数 (Scopus)

抄録

Basic residues in the S4 segments of voltage-dependent channels and leucines within the heptad repeat motif in the S4-S5 region of Shaker potassium channels have been shown to have important influences on activation. Here we have compared the relative importance for activation of S4 arginines (mutated to neutral or negative residues) in each of the four repeats of a chimeric L-type calcium channel. Significant effects on midpoint potential and time constant of activation were produced by mutations in repeats I and III but not in repeats II and IV. Leucine or isoleucine mutations in repeats I and III had the same effect on the voltage dependence of calcium channel activation as the mutations at equivalent positions in the Shaker channel, indicating that the heptad motif plays a fundamental role in channel activation.

本文言語English
ページ(範囲)2515-2523
ページ数9
ジャーナルBiophysical Journal
72
6
DOI
出版ステータスPublished - 1997 6月
外部発表はい

ASJC Scopus subject areas

  • 生物理学

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