Role of aquaporin 9 in cellular accumulation of arsenic and its cytotoxicity in primary mouse hepatocytes

Yasuhiro Shinkai, Daigo Sumi, Takashi Toyama, Toshiyuki Kaji, Yoshito Kumagai

研究成果: Article査読

44 被引用数 (Scopus)

抄録

Aquaporin (AQP) 9 is a member of the aquaglyceroporin subfamily of AQPs in the transfer of water and small solutes such as glycerol and arsenite. It is well recognized that arsenic toxicity is associated with intracellular accumulation of this metalloid. In the present study, we examined the contribution of AQP9 to the uptake of inorganic arsenite, thereby increasing arsenic-induced cytotoxicity in primary mouse hepatocytes. Pretreatment with sorbitol as a competitive inhibitor of AQP9 and siRNA-mediated knockdown of AQP9 resulted in a significant decrease of arsenite uptake in the cell and its cytotoxicity. Furthermore, overexpression of AQP9 in HEK293 cells led to the enhancement of intracellular arsenic concentration, resulting in enhanced cytotoxicity after arsenite exposure. These results suggest that AQP9 is a channel to define arsenite sensitivity in primary mouse hepatocytes.

本文言語English
ページ(範囲)232-236
ページ数5
ジャーナルToxicology and Applied Pharmacology
237
2
DOI
出版ステータスPublished - 2009 6 1
外部発表はい

ASJC Scopus subject areas

  • Toxicology
  • Pharmacology

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