In the screening of rice mutants having altered seed protein compositions, two mutant lines lacking α-globulin were selected from the M2 seeds of a white particle mutant of cv. Koshihikari irradiated with gamma-rays. The mutant character, lack of α-globulin, was found to be controlled by a single recessive gene. Northern blot analysis of endosperm RNA using the probe of α-globulin cDNA revealed that the α-globulin gene is not expressed in the α-globulin-less mutants. The mutated gene was considered to be α-globulin gene, and named glb-1(t). RFLP analysis of F2 plants between the mutant and indica cultivar 'Kasalath' using land marker probes suggested that glb-1(t) is on chromosome 5 and that the distances of glb-1(t) from the markers, C246 and X'Npb366, were 15.4cM and 30.8cM, respectively. The mutant line lacking α-globulin was expected to have a higher content of lysine in rice grains, but the lysine content of the mutant line was found not to be higher than the original cultiver. The possible use of the mutant as a breeding material is discussed.
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