Rhizobitoxine inhibition of hydrogenase synthesis in free-living Bradyrhizobium japonicum

K. Minamisawa, K. Fukai, T. Asami

研究成果: Article査読

11 被引用数 (Scopus)

抄録

Rhizobitoxine produced by Bradyrhizobium species strongly prevented derepression of hydrogenase expression in free-living Bradyrhizobium japonicum, although the toxin had no effect on the activity of cells which had already synthesized hydrogenase protein. Dihydrorhizobitoxine, a structural analog of rhizobitoxine, proved to be a less potent inhibitor of hydrogenase derepression. Rhizobitoxine did not cause cell death at a concentraation sufficient to eliminate hydrogenase expression. The large subunit of hydrogenase was not detectable with antibody after derepression in the presence of rhizobitoxine. The general pattern of proteins synthesized from 14C-labeled amino acids during derepression was not significantly different in the presence or absence of rhizohiboxine. These results indicated that rhizobitoxine inhibited hydrogenase synthesis in free-living B. japonicum. Cystathionine and methionine strongly prevented the inhibition of hydrogenase derepression by rhizobitoxine, suggesting that the inhibition involves the level of sulfur-containing amino acids in the cell.

本文言語English
ページ(範囲)4505-4509
ページ数5
ジャーナルJournal of bacteriology
172
8
DOI
出版ステータスPublished - 1990
外部発表はい

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

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