Restored interlaced volumetric imaging increases image quality and scanning speed during intravital imaging in living mice

Maina Sogabe, Masayuki Ohzeki, Koji Fujimoto, Atsuko Sehara-Fujisawa, Satoshi Nishimura

研究成果: Article査読

抄録

Dynamic intravital imaging is essential for revealing ongoing biological phenomena within living organisms and is influenced primarily by several factors: motion artifacts, optical properties and spatial resolution. Conventional imaging quality within a volume, however, is degraded by involuntary movements and trades off between the imaged volume, imaging speed and quality. To balance such trade-offs incurred by two-photon excitation microscopy during intravital imaging, we developed a unique combination of interlaced scanning and a simple image restoration algorithm based on biological signal sparsity and a graph Laplacian matrix. This method increases the scanning speed by a factor of four for a field size of 212 μm × 106 μm × 130 μm, and significantly improves the quality of four-dimensional dynamic volumetric data by preventing irregular artifacts due to the movement observed with conventional methods. Our data suggest this method is robust enough to be applied to multiple types of soft tissue.

本文言語English
論文番号e201960204
ジャーナルJournal of Biophotonics
13
5
DOI
出版ステータスPublished - 2020 5 1

ASJC Scopus subject areas

  • Chemistry(all)
  • Materials Science(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Engineering(all)
  • Physics and Astronomy(all)

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