Requirement of the Ca2+ channel β2 subunit for sympathetic PKA phosphorylation

Manabu Murakami, Feng Xu, Takayoshi Ohba, Takeshi Kobayashi, Yoshiro Inoue, Agnieszka M. Murakami, Ichiro Miyoshi, Kyoichi Ono, Noritsugu Tohse

研究成果: Article査読

抄録

Facilitation of cardiac function in response to signals from the sympathetic nervous system is initiated by the phosphorylation of L-type voltage-dependent Ca2+ channels (VDCCs) by protein kinase A (PKA), which in turn is activated by β-adrenoceptors. Among the five subunits (α1, β, α2/δ, and γ) of VDCCs, the α1 subunit and the family of β subunits are substrates for PKA-catalyzed phosphorylation; however, the subunit responsible for β-adrenergic augmentation of Ca2+ channel function has yet to be specifically identified. Here we show that the VDCC β2 subunit is required for PKA phosphorylation upon sympathetic acceleration. In mice with β2 subunit-null mutations, cardiac muscle contraction in response to isoproterenol was reduced and there was no significant increase in Ca2+ channel currents upon PKA-dependent phosphorylation. These findings indicate that within the sympathetic nervous system the β2 subunit of VDCCs is required for physiological PKA-dependent channel phosphorylation.

本文言語English
ページ(範囲)253-261
ページ数9
ジャーナルJournal of Pharmacological Sciences
145
3
DOI
出版ステータスPublished - 2021 3

ASJC Scopus subject areas

  • Molecular Medicine
  • Pharmacology

フィンガープリント 「Requirement of the Ca<sup>2+</sup> channel β<sub>2</sub> subunit for sympathetic PKA phosphorylation」の研究トピックを掘り下げます。これらがまとまってユニークなフィンガープリントを構成します。

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