Requirement of Runx1/AML1/PEBP2αB for the generation of haematopoietic cells from endothelial cells

Tomomasa Yokomizo, Minetaro Ogawa, Motomi Osato, Tomohiko Kanno, Hisahiro Yoshida, Tetsuhiro Fujimoto, Stuart Fraser, Satomi Nishikawa, Hitoshi Okada, Masanobu Satake, Tetsuo Noda, Shin Ichi Nishikawa, Yoshiaki Ito

研究成果: Article査読

147 被引用数 (Scopus)

抄録

Background: Recent studies revealing that endothelial cells derived from E8.5-E10.5 mouse embryos give rise to haematopoietic cells appear to correspond to previous histological observations that haematopoietic cell clusters are attached to the ventral aspect of dorsal aorta in such a way as if they were budding from the endothelial cell layer. Gene disruption studies have revealed that Runx1/AML1 is required for definitive haematopoiesis but not for primitive haematopoiesis, but the precise stage of gene function is not yet known. Results: We found that mice deficient in Runx1/AML1 (an α subunit of the transcription factor PEBP2/CBF) lack c-Kit+ haematopoietic cell clusters in the dorsal aorta, omphalomesenteric and umbilical arteries, as well as yolk sac vessels. Moreover, endothelial cells sorted from the embryo proper and the yolk sac of AML1-/- embryos are unable to differentiate into haematopoietic cells on OP9 stromal cells, whereas colonies of AML1-/- endothelial cells can be formed in culture. Conclusions: These results strongly suggest that the emergence of haematopoietic cells from endothelial cells represents a major pathway of definitive haematopoiesis and is an event that also occurs in the yolk sac in vivo, as suggested by earlier in vitro experiments.

本文言語English
ページ(範囲)13-23
ページ数11
ジャーナルGenes to Cells
6
1
DOI
出版ステータスPublished - 2001
外部発表はい

ASJC Scopus subject areas

  • 遺伝学
  • 細胞生物学

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