Renaturation and activation of calf prochymosin produced in an insoluble form in Escherichia coli

Yoshiyuki Kawaguchi, Norio Shimizu, Katsuhiko Nishimori, Takeshi Uozumi, Teruhiko Beppu

研究成果: Article査読

38 被引用数 (Scopus)

抄録

Calf prochymosin produced in Escherichia coli cells harboring the expression plasmids was insoluble and formed large inclusion bodies, which were solubilized by 8 M urea. The conditions allowing correct refolding of denatured prochymosin were investigated. Dialysis at pH 10 in the presence of 500 mM NaCl was found to give the maximum renaturation, and subsequent acidic treatment for autocatalytic processing of refolded prochymosin allowed almost 100% recovery of chymosin.

本文言語English
ページ(範囲)307-315
ページ数9
ジャーナルJournal of Biotechnology
1
5-6
DOI
出版ステータスPublished - 1984 12

ASJC Scopus subject areas

  • バイオテクノロジー
  • バイオエンジニアリング
  • 応用微生物学とバイオテクノロジー

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