Renal expression of the essential genes associated with sodium transport following a total proctocolectomy in rats

Kouhei Fukushima, Shun Sato, Hiroo Naito, Yuji Funayama, Chikashi Shibata, Iwao Sasaki

研究成果: Article

3 引用 (Scopus)

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The water and electrolyte balance is regulated by the renin-angiotensin- aldosterone system in the kidney. We previously reported that the levels of circulating aldosterone dramatically increased following a total proctocolectomy in rats. However, there is no direct evidence regarding whether renal adaptation is accelerated by the induction of aldosterone-associated molecules. To explore this question, Sprague-Dawley rats underwent total proctocolectomies and then were killed 8 weeks later. We investigated the renal expression of 11β-hydroxysteroid dehydrogenase type 2, the α-, β-, and γ-subunits of the epithelial sodium channel, and the α1- and β1-subunits of Na+,K+-ATPase mRNAs because those molecules are responsible for the aldosterone specificity for mineralocorticoid receptor, amiloride-sensitive sodium absorption, and sodium extrusion from distal tubules, respectively. A Northern blot analysis demonstrated the kidney to exhibit mRNA induction for all of these molecules, thus supporting the idea that renal adaptation following a total proctocolectomy depends, at least in part, upon the molecular induction which is principally regulated by circulating aldosterone.

元の言語English
ページ(範囲)502-504
ページ数3
ジャーナルSurgery today
35
発行部数6
DOI
出版物ステータスPublished - 2005 6 1

ASJC Scopus subject areas

  • Surgery

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