Regulatory DNA elements localized remotely upstream from the drug-metabolizing cytochrome P-450c gene

Atsuko Ujisawa-Sehara, Kazuhiro Ogawa, Chikako Nishi, Yoshiaki Fujii-Kuriyama

    研究成果: Article査読

    97 被引用数 (Scopus)

    抄録

    We have investigated regulatory DNA elements in the expression of the drug metabolizing P-450c gene of rats. Aftar combining the 5′ flanking and upstream untranslated regions of the isolated P-450c gene with structural gene for chloramphenicol acetyltransferase (CAT), the fusion genes were transfected into cultured cells (Kepa-1 and L cells) for the assay of transient expression of CAT activity. CAT activity was expressed inducibly in response to 3-methylcholanthrene only in Kepa-1 cells. At least three regions containing regulatory DNA elements were indentified; one, which is present in the sequence from -44b to -0.2kb immediately upstream of the TATA box, functions in the basal level of transcription, and the other two which were located in the sequence from -0.8kb to -1.0kb and from -1.0kb to -6.3kb, enhance in combination, transcription in response to inducers in a manner independent of their orientation.

    本文言語English
    ページ(範囲)1465-1477
    ページ数13
    ジャーナルNucleic acids research
    14
    3
    DOI
    出版ステータスPublished - 1986 2 11

    ASJC Scopus subject areas

    • 遺伝学

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