Regulation of synaptotagmin gene expression during ascidian embryogenesis

You Katsuyama, Jun Matsumoto, Toshiaki Okada, Yukio Ohtsuka, Ling Chen, Haruo Okado, Yasushi Okamura

    研究成果: Article査読

    28 被引用数 (Scopus)

    抄録

    The ascidian embryo, a model for the primitive mode of chordate development, rapidly forms a dorsal nervous system which consists of a small number of neurons. Here, we have characterized the transcriptional regulation of an ascidian synaptotagmin (syt) gene to explore the molecular mechanisms underlying development of synaptic transmission. In situ hybridization showed that syt is expressed in all neurons described in previous studies and transiently in the embryonic epidermis. Neuronal expression of syt requires induction from the vegetal side of the embryo, whereas epidermal expression occurs autonomously in isolated ectodermal blastomeres. Introduction of green fluorescent protein reporter gene constructs into the ascidian embryos indicates that a genomic fragment of the 3.4-kb 5′ upstream region contains promoter elements of syt gene. Deletion analysis of the promoter suggests that syt expression in neurons and in the embryonic epidermis depends on distinct cis-regulatory regions.

    本文言語English
    ページ(範囲)293-304
    ページ数12
    ジャーナルDevelopmental Biology
    244
    2
    DOI
    出版ステータスPublished - 2002 4月 15

    ASJC Scopus subject areas

    • 分子生物学
    • 発生生物学
    • 細胞生物学

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