Regulation of PPARγ transcriptional activity in 3T3-L1 adipocytes

Masaki Watanabe, Kouichi Inukai, Hideki Katagiri, Takuya Awata, Yoshitomo Oka, Shigehiro Katayama

研究成果: Article査読

38 被引用数 (Scopus)

抄録

PPARγ is a member of the nuclear hormone receptor superfamily and functions as a transcriptional regulator of genes linked to adipogenesis and lipid metabolism. The regulation of PPARγ activity by insulin signaling molecules in adipocytes has yet to be clarified. Therefore, it is important to measure endogenous PPARγ transcriptional activities in response to various stimuli in adipocytes. Herein, with a transcription reporter assay using recombinant adenovirus vectors expressing PPRE (PPAR responsive elements)-reporter genes, we established a novel system for measuring endogenous PPARγ transcriptional activity in 3T3-L1 adipocytes. By means of this system, a marked increase (8.5-fold) in PPARγ transcriptional activity was detected after treatment with 10-6M pioglitazone, a thiazolidinedione (TZD), indicating that this system can measure PPARγ activity accurately. Furthermore, MAPK activation, achieved by overexpressing constitutively activated MEK1, inhibited PPARγ transcriptional activity. In contrast, treatment with PKA stimulators markedly increased PPARγ activity. Interestingly, PI 3-kinase overexpression resulted in a marked decrease in PPARγ activity. These observations have important implications for understanding the regulation of PPARγ transcriptional activity.

本文言語English
ページ(範囲)429-436
ページ数8
ジャーナルBiochemical and biophysical research communications
300
2
DOI
出版ステータスPublished - 2003 1 10
外部発表はい

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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