Regulation of nerve growth factor and nerve growth factor receptor production by NMDA in C6 glioma cells

Takehiko Amano, Tohru Yamakuni, Noriko Okabe, Reiko Kuwahara, Fumiko Ozawa, Fumio Hishinuma

研究成果: Article査読

25 被引用数 (Scopus)

抄録

The synthesis of nerve growth factor (NGF) and nerve growth factor receptor (NGFR) were studied in a C6 glioma cell line by Northern blot hybridization. In responseto a glutamate agonist N-methyl-d-aspartic acid (NMDA), NGF mRNA increased by up 2-fold after 4-12 h of culture. The non-NMDA receptor agonists, quisqualate and kainate, did not induce any increase of NGF mRNA, and kainate actually produced a decrease. The increase in NGF mRNA in response to NMDA was dose-dependent at 1, 5 and 10 μM. NGF receptor (NGFR) mRNA showed changes in expression which were similar to those for NGF mRNA, but were less marked. The specific glutamate antagonist 2-aminophosphonovaleric acid (APV) blocked the increase of NGF mRNA produced by NMDA. In the absence of Ca2+, an increase of NGF mRNA was still observed but in the presence of 1 mM ethylglycol-bis-(β-aminoethyl ether) N,N′-tetraacetic acid (EGTA), NGF mRNA production abolished. The mechanism producing an increase in NGF mRNA by NMDA may be mediated by cyclic AMP since intracellular cyclic AMP and NGF mRNA levels both increased following treatment with NMDA or dibutyryl cyclic AMP.

本文言語English
ページ(範囲)35-42
ページ数8
ジャーナルMolecular Brain Research
14
1-2
DOI
出版ステータスPublished - 1992 6
外部発表はい

ASJC Scopus subject areas

  • 分子生物学
  • 細胞および分子神経科学

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