TY - JOUR
T1 - Regulation of cementoblast function by P. gingivalis lipopolysaccharide via TLR2
AU - Nemoto, E.
AU - Darveau, R. P.
AU - Foster, B. L.
AU - Nogueira-Filho, G. R.
AU - Somerman, M. J.
PY - 2006/8
Y1 - 2006/8
N2 - Although cementoblasts express Toll-like receptors (TLR)-2 and -4, little is known regarding the possible participation of cementoblasts in the inflammatory response. We investigated the effects of Porphyromonas gingivalis lipopolysaccharide (LPS), tetra- and penta-acylated lipid A species (designated PgLPS1435/1449 and PgLPS1690, respectively), on gene expression of osteoclastogenesis-associated molecules in murine cementoblasts. Real-time quantitative RT-PCR analysis revealed that receptor activator of NF-κB ligand (RANKL), interleukin-6, Regulated on activation, normal T-cell expressed, and secreted (RANTES), macrophage inflammatory protein-1α, and monocyte chemoattractant protein-1 were rapidly and dramatically induced upon stimulation with PgLPS1690, but only slightly induced with PgLPS1435/1449. Osteoprotegerin, which was expressed constitutively, was not altered significantly. ELISA demonstrated synthesis of corresponding proteins. PgLPS1690 significantly induced transcripts for NF-κB, and this activation was inhibited by pre-treatment with anti-TLR-2 but not with TLR-4 antibodies. These results suggest that cementoblasts participate in the recruitment of osteoclastic precursor cells by up-regulation of chemokines/cytokines.
AB - Although cementoblasts express Toll-like receptors (TLR)-2 and -4, little is known regarding the possible participation of cementoblasts in the inflammatory response. We investigated the effects of Porphyromonas gingivalis lipopolysaccharide (LPS), tetra- and penta-acylated lipid A species (designated PgLPS1435/1449 and PgLPS1690, respectively), on gene expression of osteoclastogenesis-associated molecules in murine cementoblasts. Real-time quantitative RT-PCR analysis revealed that receptor activator of NF-κB ligand (RANKL), interleukin-6, Regulated on activation, normal T-cell expressed, and secreted (RANTES), macrophage inflammatory protein-1α, and monocyte chemoattractant protein-1 were rapidly and dramatically induced upon stimulation with PgLPS1690, but only slightly induced with PgLPS1435/1449. Osteoprotegerin, which was expressed constitutively, was not altered significantly. ELISA demonstrated synthesis of corresponding proteins. PgLPS1690 significantly induced transcripts for NF-κB, and this activation was inhibited by pre-treatment with anti-TLR-2 but not with TLR-4 antibodies. These results suggest that cementoblasts participate in the recruitment of osteoclastic precursor cells by up-regulation of chemokines/cytokines.
KW - Cementoblast
KW - Lipopolysaccharide
KW - Osteoclastogenesis
KW - Porphyromonas gingivalis
KW - Toll-like receptor
UR - http://www.scopus.com/inward/record.url?scp=33748092341&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=33748092341&partnerID=8YFLogxK
U2 - 10.1177/154405910608500809
DO - 10.1177/154405910608500809
M3 - Article
C2 - 16861291
AN - SCOPUS:33748092341
VL - 85
SP - 733
EP - 738
JO - Journal of Dental Research
JF - Journal of Dental Research
SN - 0022-0345
IS - 8
ER -