Regulation of Cell Volume by Nanosecond Pulsed Electric Fields

Qi Yang, Shinji Kajimoto, Yuki Kobayashi, Hirotsugu Hiramatsu, Takakazu Nakabayashi

研究成果: Article査読

2 被引用数 (Scopus)


Stimulation of cells by nanosecond pulsed electric fields (nsPEFs) has attracted attention as a technology for medical applications such as cancer treatment. nsPEFs have been shown to affect intracellular environments without significant damage to cell membranes; however, the mechanism underlying the effect of nsPEFs on cells remains unclear. In this study, we constructed electrodes for applying nsPEFs and analyzed the change in volume of a single cell due to nsPEFs using fluorescence and Raman microscopy. It was shown that the direction of the change depended on the applied electric field; expansion due to the influx of water was observed at high electric field, and cell shrinkage was observed at low electric field. The change in cell volume was correlated to the change in the intracellular Ca2+concentration, and nsPEFs-induced shrinking was not observed when the Ca2+-free medium was used. This result suggests that the cell shrinkage is related to the regulatory volume decrease where the cell adjusts the increase in intracellular Ca2+concentration, inducing the efflux of ions and water from the cell.

ジャーナルJournal of Physical Chemistry B
出版ステータスPublished - 2021 9月 30

ASJC Scopus subject areas

  • 物理化学および理論化学
  • 表面、皮膜および薄膜
  • 材料化学


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