Reduced susceptibility to a tobacco bushy top virus malawi isolate by loss of function in host eif(Iso)4e genes

Hisashi Udagawa, Kazuharu Koga, Akira Shinjo, Hiroyasu Kitashiba, Yoshimitsu Takakura

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Tobacco bushy top disease (TBTD) is a viral disease of tobacco (Nicotiana tabacum L.) caused by mixed infection of Tobacco bushy top virus or Ethiopian tobacco bushy top virus and a helper virus. Despite its damage to tobacco, practical genetic resources for disease resistance have not been found. Here, we report that a mutation of tobacco eIF(iso)4E genes (eIF(iso)4E-S and eIF(iso)4E-T), which encode eukaryotic translation initiation factors, confers resistance (reduced susceptibility) to TBTD caused by a virus from Malawi (designated as tobacco bushy top virus Malawi isolate, TBTV-MW). RNAi lines in which eIF(iso)4E genes were silenced showed reduced susceptibility to TBTV-MW. We also tested chemically-induced single (eIF(iso)4E-S or eIF(iso)4E-T) and double (eIF(iso)4E-S and eIF(iso)4E-T) nonsense mutants for resistance to TBTV-MW. Suppression of eIF(iso)4E-S showed reduced susceptibility, and the resistance of the double mutant tended to be even stronger. eIF(iso)4E mutants also showed reduced susceptibility to TBTV-MW transmitted by aphids. To the best of our knowledge, the eIF(iso)4E-S mutant is the first genetic resource for TBTD resistance breeding in tobacco.

本文言語English
ページ(範囲)313-320
ページ数8
ジャーナルBreeding Science
70
3
DOI
出版ステータスPublished - 2020

ASJC Scopus subject areas

  • 農業および作物学
  • 遺伝学
  • 植物科学

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