Redox sensor proteins for highly sensitive direct imaging of intracellular redox state

Kazunori Sugiura, Takeharu Nagai, Masahiro Nakano, Hiroshi Ichinose, Takakazu Nakabayashi, Nobuhiro Ohta, Toru Hisabori

研究成果: Article査読

28 被引用数 (Scopus)

抄録

Intracellular redox state is a critical factor for fundamental cellular functions, including regulation of the activities of various metabolic enzymes as well as ROS production and elimination. Genetically-encoded fluorescent redox sensors, such as roGFP (Hanson, G. T., et al. (2004)) and Redoxfluor (Yano, T., et al. (2010)), have been developed to investigate the redox state of living cells. However, these sensors are not useful in cells that contain, for example, other colored pigments. We therefore intended to obtain simpler redox sensor proteins, and have developed oxidation-sensitive fluorescent proteins called Oba-Q (oxidation balance sensed quenching) proteins. Our sensor proteins derived from CFP and Sirius can be used to monitor the intracellular redox state as their fluorescence is drastically quenched upon oxidation. These blue-shifted spectra of the Oba-Q proteins enable us to monitor various redox states in conjunction with other sensor proteins.

本文言語English
ページ(範囲)242-248
ページ数7
ジャーナルBiochemical and biophysical research communications
457
3
DOI
出版ステータスPublished - 2015 2 13

ASJC Scopus subject areas

  • 生物理学
  • 生化学
  • 分子生物学
  • 細胞生物学

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