TY - JOUR
T1 - Redox phospholipid polymer microparticles as doubly functional polymer support for immobilization of enzyme oxidase
AU - Lin, Xiaojie
AU - Konno, Tomohiro
AU - Takai, Madoka
AU - Ishihara, Kazuhiko
PY - 2013/2/1
Y1 - 2013/2/1
N2 - We prepared redox phospholipid polymer microparticles for immobilizing an enzyme in order to maintain activity for a long time and obtain highly effective electron transfer to a gold substrate as an electrode. To achieve these double functions, an amphiphilic redox phospholipid polymer, poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-nitrophenyloxycarbonyl oligo(ethylene glycol) methacrylate (MEONP)-co-vinylferrocene (VFc)) (PMBNF) was synthesized. The polystyrene (PS) microparticles were modified by employing a simple solution dip-coating technique to form the PMBNF layer on the surface. As one of the model enzyme oxidases, a glucose oxidase (GOx) was immobilized on the PMBNF/PS microparticles by the reaction between the MEONP units in the PMBNF layer and the amino group in the GOx. The activity of immobilized GOx is maintained well; for example, activity of more than 80% of the initial activity was observed even after storage at both 4°C and 25°C (ionic strength: 0.10mol/L, phosphate buffer solution, pH 7.0) for at least one month. The GOx/PMBNF/PS microparticles were arrayed on a gold substrate in a monolayer, and then, crosslinked to each other with a polymeric diamine compound. The PMBNF/PS microparticles demonstrated an efficient electron transfer from immobilized GOx to the gold surface. From these results, we concluded that the PMBNF layer on the PS microparticles possessed double functions such as stable enzyme immobilization ability and efficient electron transfer ability.
AB - We prepared redox phospholipid polymer microparticles for immobilizing an enzyme in order to maintain activity for a long time and obtain highly effective electron transfer to a gold substrate as an electrode. To achieve these double functions, an amphiphilic redox phospholipid polymer, poly(2-methacryloyloxyethyl phosphorylcholine-co-n-butyl methacrylate-co-p-nitrophenyloxycarbonyl oligo(ethylene glycol) methacrylate (MEONP)-co-vinylferrocene (VFc)) (PMBNF) was synthesized. The polystyrene (PS) microparticles were modified by employing a simple solution dip-coating technique to form the PMBNF layer on the surface. As one of the model enzyme oxidases, a glucose oxidase (GOx) was immobilized on the PMBNF/PS microparticles by the reaction between the MEONP units in the PMBNF layer and the amino group in the GOx. The activity of immobilized GOx is maintained well; for example, activity of more than 80% of the initial activity was observed even after storage at both 4°C and 25°C (ionic strength: 0.10mol/L, phosphate buffer solution, pH 7.0) for at least one month. The GOx/PMBNF/PS microparticles were arrayed on a gold substrate in a monolayer, and then, crosslinked to each other with a polymeric diamine compound. The PMBNF/PS microparticles demonstrated an efficient electron transfer from immobilized GOx to the gold surface. From these results, we concluded that the PMBNF layer on the PS microparticles possessed double functions such as stable enzyme immobilization ability and efficient electron transfer ability.
KW - 2-Methacryloyloxyethyl phosphorylcholine polymer
KW - Electron transfer mediator
KW - Enzyme immobilization
KW - Polymer microparticles
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U2 - 10.1016/j.colsurfb.2012.09.024
DO - 10.1016/j.colsurfb.2012.09.024
M3 - Article
C2 - 23107964
AN - SCOPUS:84867762955
VL - 102
SP - 857
EP - 863
JO - Colloids and Surfaces B: Biointerfaces
JF - Colloids and Surfaces B: Biointerfaces
SN - 0927-7765
ER -