Redox cycling-based electrochemical reporter gene assay for single cells using a scanning electrochemical microscope-microwell system

Hiroki Ida, Kosuke Ino, Junya Suzuki, Yasufumi Takahashi, Hitoshi Shiku, Tomokazu Matsue

研究成果: Article査読

2 被引用数 (Scopus)

抄録

Single-cell analyses are important for providing new insights into cellular biology. Here we report an electrochemical reporter gene assay for single cells using a scanning electrochemical microscope (SECM)-microwell system. Each microwell trapped a single cell that synthesized a reporter protein, secreted alkaline phosphatase (SEAP). The SEAP catalyzed the hydrolysis of p-aminophenyl phosphate to p-aminophenol (PAP). A disk electrode in the SECM was positioned above the microwell and monitored the oxidation currents of PAP derived from SEAP. In addition, ring electrodes were prepared on the microwell device to induce redox cycling between the ring and disk electrodes, thus amplifying the electrochemical signals from the reporter protein. The redox cycling-based electrochemical reporter gene assay is useful for single-cell analyses.

本文言語English
ページ(範囲)308-311
ページ数4
ジャーナルElectrochemistry
84
5
DOI
出版ステータスPublished - 2016 5

ASJC Scopus subject areas

  • 電気化学

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