@article{33df1c72dca14784811bc09570407d8d,
title = "Redox-coupled structural changes in nitrite reductase revealed by serial femtosecond and microfocus crystallography",
abstract = "Serial femtosecond crystallography (SFX) has enabled the damage-free structural determination of metalloenzymes and filled the gaps of our knowledge between crystallographic and spectroscopic data. Crystallographers, however, scarcely know whether the rising technique provides truly new structural insights into mechanisms of metalloenzymes partly because of limited resolutions. Copper nitrite reductase (CuNiR), which converts nitrite to nitric oxide in denitrification, has been extensively studied by synchrotron radiation crystallography (SRX). Although catalytic Cu (Type 2 copper (T2Cu)) of CuNiR had been suspected to tolerate X-ray photoreduction, we here showed that T2Cu in the form free of nitrite is reduced and changes its coordination structure in SRX. Moreover, we determined the completely oxidized CuNiR structure at 1.43 {\AA} resolution with SFX. Comparison between the high-resolution SFX and SRX data revealed the subtle structural change of a catalytic His residue by X-ray photoreduction. This finding, which SRX has failed to uncover, provides new insight into the reaction mechanism of CuNiR.",
keywords = "X-ray free-electron laser, copper, electron transfer, enzyme, serial femtosecond crystallography",
author = "Yohta Fukuda and Tse, {Ka Man} and Mamoru Suzuki and Kay Diederichs and Kunio Hirata and Takanori Nakane and Michihiro Sugahara and Eriko Nango and Kensuke Tono and Yasumasa Joti and Takashi Kameshima and Changyong Song and Takaki Hatsui and Makina Yabashi and Osamu Nureki and Hiroyoshi Matsumura and Tsuyoshi Inoue and So Iwata and Eiichi Mizohata",
note = "Funding Information: This work was supported by the X-ray Free-Electron Laser Priority Strategy Program of the Ministry of Education, Culture, Sports, Science and Technology in Japan (MEXT), the Grant-in-Aid for Scientific Research on Innovative Areas from MEXT, the Grantin-Aid for Japan Society for the Promotion of Science (JSPS) Research Fellows (Grant no. 254626), and the JSPS KAKENHI (Grant no. 15K18487). Publisher Copyright: {\textcopyright} 2016 The Authors 2016. Published by Oxford University Press on behalf of the Japanese Biochemical Society.",
year = "2016",
month = may,
day = "1",
doi = "10.1093/jb/mvv133",
language = "English",
volume = "159",
pages = "527--538",
journal = "Journal of Biochemistry",
issn = "0021-924X",
publisher = "Oxford University Press",
number = "5",
}