Reconstitution of biosynthetic machinery of fungal polyketides: Unexpected oxidations of biosynthetic intermediates by expression host

Ryuya Fujii, Takahiro Ugai, Hirofumi Ichinose, Mayumi Hatakeyama, Takuto Kosaki, Katsuya Gomi, Isao Fujii, Atsushi Minami, Hideaki Oikawa

研究成果: Article査読

17 被引用数 (Scopus)

抄録

Reconstitution of whole biosynthetic genes in Aspergillus oryzae has successfully applied for total biosynthesis of various fungal natural products. Heterologous production of fungal metabolites sometimes suffers unexpected side reactions by host enzymes. In the studies on fungal polyketides solanapyrone and cytochalasin, unexpected oxidations of terminal olefin of biosynthetic intermediates were found to give one and four by-products by host enzymes of the transformants harboring biosynthetic genes. In this paper, we reported structure determination of by-products and described a simple solution to avoid the undesired reaction by introducing the downstream gene in the heterologous production of solanapyrone C.

本文言語English
ページ(範囲)426-431
ページ数6
ジャーナルBioscience, Biotechnology and Biochemistry
80
3
DOI
出版ステータスPublished - 2016

ASJC Scopus subject areas

  • バイオテクノロジー
  • 分析化学
  • 生化学
  • 応用微生物学とバイオテクノロジー
  • 分子生物学
  • 有機化学

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